Identification of Genes Controlling Seedling Stage Traits in Iranian Rice Recombinant Lines under Drought Stress Conditions

Rice is one the most important crops in Iran and worldwide. Abiotic stresses including drought, restrict rice production. In order to saturation of linkage map in recombinant lines population caused by Sepidroud × Anbarbou crosses, an experiment was conducted using 96 recombinant lines and 40 ISSR markers at Gonbad Kavous University. 96 Recombinant lines were plant under hydroponic conditions for mapping of traits related to drought stress. Shoot weight, root weight, biomass, genetic score, leaf area, shoot length, root length and root thickness were recorded. Linkage map covered 1709.29 cM of rice genome. Four QTLs mapped on chromosomes 3(2 QTLs) and 7 (2QTLs) in drought. qDRW-3 and qDBM-3 with LOD=2.518 and 2.727 had positive additive effects on root weight and biomass, respectively and explained 11.4 and 12.3% of phenotypic variation. The results of present work can be used for improvement of drought tolerance in rice seedlings after determining marker validation.