The Effect of Seed Inoculation with Different Bacterial Strains on some Acclimation Metabolites, Yield and Antioxidant Properties of Dill (Anethum graveolens L.) under Water Deficit Stress

IntroductionThis study was aimed to study effects of different bacterial strains on proline, chlorophyll and carbohydrate content under water shortage conditions in the field.
Materials and methodsA split plot experiment was used based on a randomized complete design in three replicates, with stress in three levels of 40, 80 and 120 mm evaporation from pan and nine different bacterial strains as the main factor and the sub-factor, respectively.
Results and DiscussionThe results showed that application of different bacterial strains and stress influenced the quantity of soluble carbohydrate in the leaves and proline. The highest amount of carbohydrate was obtained from seed inoculation of pseudomonas 169 along with 120 mm evaporation from the pan. Highest antioxidan properties by 89 mg/ml obtained in Azospirillium off and irrigation after 120 mm evaporation from the pan and lowest observed by 15.3 mg/ml in no bacterial treatment and irrigation after 40 mm evaporation from the pan. Also highest essential oil yield by 4 gr observed in herbasilus and 120 mm evaporation from the pan and lowest observed in Pseudomonas 168 and 40 mm evaporation from the pan by 0.85 gr. In this investigation in low and high grought stress using bacterial strains increased prolin and carbohydrate production than control. So using azeto 5 and azospirillium 21 in moderate drought and azeto 5 and herbasillus in sever drought stress can increase essential oil yield. These results provide enough evidence as to application of biological fertilizers brings about enhancements to the quality and quantity of dill. This could increase the plant oil yield under water shortage is and moderate conditions compared to the control.
ConclusionsThe use of appropriate strains of bacteria such as Azatobacter5 and Azospirllium 21 can in condition of moderate and Azatobacter 5, pseudomonas 169 under stress condition, in medicinal plant to increase oil production.