Comparison of optimized DNA extraction from mold Aspergillus, Penicillium and Rhizopus of tomato paste
Author(s):
Abstract:
In this study, the optimized method for DNA extraction from Aspergillus niger, Rhizopus oryzae and Penicillium chrysogenom in tomato paste were used. These methods are presented in accordance with the Al-Samarai That changes in the concentration of extraction buffer and some stages it were given to the optimization method. Mold spores of at different levels (101, 103 and 105 CFU/g) were added to the tomato paste. After mycelium growth DNA extraction was perfomed by this methods. This method in terms of quality and quantity of DNA extracted was studied using spectrophotometer with NanoDrop. The extracted DNA using gel electrophoresis for protein contamination and absence of smear were evaluated. In order to investigate The contamination and no presecnce of PCR inhibitors determined with PCR that performed forward and reverse primers. Results of gel electrophoresis and PCR assay showed that This method is suitable for DNA extraction is the mold of tomato paste.
Keywords:
Language:
Persian
Published:
Food Science and Technology, Volume:14 Issue: 6, 2017
Page:
1
magiran.com/p1733579
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!