Investigation of anti-radical properties of lignin extracted from plant species by two methods of MWL and DL

Today due to known anti-radical activity in plants extracted compounds and its derives was regarded to adding those to food and biological systems. In present paper investigated radical scavenging activity of lignin (as a non-carbohydrate poly-phenol) was extracted from different plant resources. TAPPI standard, T222om-88h was used In order to determination of total lignin. The amount of lignin in abies species was much more than others. Lignin was prepared from three species of bagas (Herbaceous), abies (bornmuelleriana) (Needle-leaf) and poplar (populous euroamerica) (Broadleaved) by two process of Milled Wood Lignin (MWL) and Acidic Dioxan Lignin (DL).the extraction efficiency of the DL was higher than MWL method in all species. The radical scavenging activity and phenolic compound were determinate by stable DPPH free radical and Folin- ciocalteu reagent, Respectively. The results showed that lignin extracted from cane sugar bagasse in both MWL and DL methods has more radical scavenging activity in compare with other specimens. In all of samples DL has better anti-radical activity than MWL extraction method. The lowest and highest amount of IC50Were attributed to bagasse (278.7 ±1,46) and poplar (682.2 ±0.71) , respectively.