Phylogenetic analysis of new Iranian Fig badnavirus-1 isolates based on their movement protein gene sequence

Fig mosaic disease is considered as the most common fig viral disease, which occurs worldwide. Fig badnavirus-1 (FBV-1) is one of the most important viruses involved in this viral disease. In the present research, phylogenetic relationship between the Iranian and other detected FBV-1 isolates was studied. A genomic fragment with an expected size about 1090 bp was amplified from the movement protein coding region of twelve virus isolates using specific primers. Amplified fragments were cloned in plasmid pTZ57R/T, and sequenced. Obtained sequences were phylogenetically compared with the corresponding isolates available in the GenBank after multiple alignments. The phylogenetic tree was drawn for the FBV-1 isolates based on the movement protein gene sequence. The studied isolates were categorized into two distinct phylogroups in which Iranian isolates were separated into two different subgroups in group I with 99% identity at the nucleotide level. Phylogeny on the basis of the amino acid sequences categorized isolates with 98-99٪ identity into three different groups. According to the results of this study, it could be concluded that genetic variation between Iranian isolates is less than that of American isolates, and additionally the genetic variation between the isolates is affected by the geographical condition.