Frequency of rhlR, rhlI and lasR Genes in Pseudomonas aeruginosa Isolated from Urinary Tract Infections. Tehran, 2016

Pseudomonas aeruginosa is the most common bacterial of Urinary tract infection that is important for appropriate treatment. This bacteria has two complete system quorum sensing that are activated pathogenic genes. In this study was isolated antibiotic resistante Pseudomonas aeruginosa from Urinary Tract Infection. Then by molecular analysis detected rhlR, rhlI and lasR genes as genetic marker for identification of Pseuodomonas aeroginosa.
Materials and methods- 150 urine samples were collected. Pseudomonas aeruginosa was isolated from urine by biochemical tests. Antibiogram was performed by disk diffusion method. DNA was extracted from resistant isolates. Then rhlR, rhlI and lasR genes were found by PCR.
Results- 23 strains of Pseudomonas aeruginosa were isolated from urine samples. The most strains of Pseudomonas aeruginosa were resistant to ceftazidime and meropenem, while the least resistance were observed for ciprofloxacin and piperacillin-tazobactam. Frequency of rhlR, rhlI and rhlR genes were in 11, 2 and 15 strains, respectively.
Conclusion- Results showed that rhlR, rhlI and lasR genes were existed in resistant Pseudomonas aeruginosa. The defectives of quorum sensing genes reduces the susceptibility to antibiotics. In this study just rhlR, rhlI and lasR genes were analyzed and for correlation determining require a full genetic panel.