Preparation and thickness profile of endothelial keratoplasty lenticules from donated whole eyes with previous photorefractive keratectomy

To describe the preparation and thickness profiles of endothelial keratoplasty lenticules harvested from eyes with previous photorefractive keratectomy (PRK).
Donor whole eyes that underwent PRK were subjected to microkeratome‑assisted dissection for Descemet stripping automated endothelial keratoplasty. Specular microscopy and Visante optical coherence tomography were performed on precut corneas. Endothelial cell indices and thickness profiles of endothelial keratoplasty lenticules were statistically analyzed. Postoperative reports for transplanted lenticules were recorded.
Over a 6‑month period, 2,929 whole eyes from 1,471 donors were screened for PRK. Twenty‑five (0.85%) eyes from 14 donors were diagnosed with disciform haziness due to prior PRK and were used uneventfully for preparation of endothelial keratoplasty lenticules. Mean endothelial cell count was 3164.6 ± 311.0/mm2 and mean central posterior lenticule thickness was 128 ± 34 µm. Posterior lenticules revealed an increase in thickness from the central to peripheral cornea (mean increase of 26.2 µm at pericentral and 90.4 µm at peripheral locations). Mean increase in thickness was statistically different between two peripheral locations (74.5 µm vs. 108.1 µm, P = 0.047). Postoperative reports of transplanted lenticules revealed no posterior flap detachment or loss of clarity at least three months after the surgery.
PRK donor whole eyes are potential sources for preparation of microkeratome‑assisted thin endothelial keratoplasty lenticules with a high endothelial cell count. Although an asymmetric and significant increase in thickness was present at the peripheral cornea, neither attachment nor clarity of transplanted lenticules was affected by variations in thickness of precut corneas.