Comparative Analysis of Outcome of Three Kinds of Auto Fat Graft Including Free Fat, Dermo-Fat and Reverse Dermo-Fat Graft

Introduction & There is rising variety of fillers used in aesthetic surgery for skin augmentation and enhancement. The natural fillers include free fat graft, derom-fat graft and reverse dermo-fat graft (dermis down and fat up). There is not a unified opinion on comparative longevity for these grafts among authors. The purpose of this article is to find out the out the come of these grafts in the animal model.
The study was performed at St. Fatima hospital animal laboratory on 12 Spraugue Dawly male rats who weighed 300 to 350 grams. Three forms of auto fat graft including free fat, dermo-fat and reverse dermo-fat was applied and after two months animals were sacrificed. The specimens were weighed before and after grafting with precise digital scale. All specimens stained with H&E dye and studied for the amount of fibroblast proliferation, collagen bundles, necrosis, neovascularization and eosinophil and giant cell infiltration.
We found statistically no significant difference of the pre and post graft weight changes between these three groups. We also couldn’t find and significant differences between the amount of fibroblast proliferation, collagen bundles, necrosis, neovascularization and eosinophil and giant cell infiltration.
These findings show that free fat can be used as filler with no need to be combined with dermis. On the other hand we found out that derom-fat take and outcome is not dependent on the position of dermis whether to be over or under the fat graft.