Production of Doubled haploid lines in rapeseed (Brassica napus L.) via microspore embryogenesis and Evaluation of general combining ability of the lines for morphological and yield traits

In this research, Hayola F1 hybrids were used to produce rapeseed doubled haploid lines using microspore embryogenesis. To study general combining ability (GCA) of, the induced doubled haploid (DH) rapeseed lines, a top cross analysis was conducted using 28 doubled haploid lines and top cross parent of Hayola 420. Produced hybrids of doubled haploid lines × Hayola 420 were sown in research farm in 2015 growing season. Plant height, number of pods per branch and sub branches, number of seeds per pod, pod length, number of sub branches, length of main branch, 1000-seeds weight, single plant yield, number of days to flowering, number of days to seeding, number of days to physiological maturity, and oil yield were recorded in all top cross progeny to investigate GCA of DH lines. Results of analysis of variance showed a significant difference among all top cross hybrids for all investigated traits at 1% probability level. Based on means comparison analysis using multiple range Duncan test at 1% probability level, top cross hybrids of DH1, DH8, DH10, DH11, DH13, and DH21 were more differ than other top cross hybrids for all investigated characteristics. The highest mean of seed yield and oil yield was related to the top cross progeny of DH21 × Hayola 420. Results of top cross analysis showed that the highest positive and significant GCAs for single plant seed yield, number of pods per plant, and 1000-seeds weight were corresponded to DH1, DH10, and DH21, therefore these three DH lines can be used as elite parental lines in future breeding programs of rapeseed.