Investigation of the Effect of Inhalation of Magnetic Iron Oxide Nanoparticles on the Expression of P53 in the Testis Tissue of Balb/C Mice by Immunohistochemically Staining

Aim: The present study was designed to investigate the effects of chitosan/poly vinyl alcohol scaffold incorporated by NRBE (neonatal rat brain extract) on neuronal differentiation of P19 EC (embryonal carcinoma) stem cells.
Material and In order to induce neuronal phenotype, P19-derived embryonic carcinoma stem cells were cultured on a rat chitosan/poly (vinyl alcohol) scaffold with newborn rat brain extracts. To evaluate the survival potential, migration and differentiation of the three-dimensional culture cells, these cells were grafted to the developing central nervous system of the chick embryo. Finally, the transplanted cells were detected using specific staining and immunofluorescence methods.
Cresyl-Violet specific staining confirmed the neuronal phenotype of the transplantation cells. Also, the expression of specific neural protein, synaptophysin, was shown using the immunofluorescence process.
The results of this study showed that P19 embryonic carcinoma stem cells can be used as a source of pluripotent cells in transplantation studies in order to investigate cellular and molecular aspects of developmental and cellular differentiation.