Simulated microgravity effects on cell cycle in adipose derived stem cells

In recent years, biophysical forces especially microgravity increasingly has been used for cell manipulation, in particular cell differentiation and tissue engineering. Microgravity, as a mechanical factor, has been demonstrated to stimulate cell proliferation and differentiation. In this study, we investigated the impact of simulated microgravity condition generated by one-axis clinostat, on cell cycle and apoptosis rate of adipose derived stem cells (ADSCs).
Material and After isolation and characterization of human ADSCs, they were exposed to simulated microgravity condition generated by one-axis clinostat, for 3 days. After 3 days of clinorotation, control and microgravity samples were stained with PI, then, we evaluated cell cycle and apoptosis rate.
Isolated cells expressed mesenchymal markers and were able to differentiate into adipocytes and osteocytes. Our results showed that 3 days of simulated microgravity induced by one-axis clinostat had no statistically significant effects on the cell cycle and apoptosis rate.
Previous studies have shown that simulated microgravity induced by 3-axis clinostat or RPM altered cell cycle and increased apoptosis rate. Based on our data, it seems that shear stress generated by one-axis clinostat is much less than that generated by three-axis clinostat. Therefore, we recommend use of one-axis clinostat for evaluation of cell differentiation and cell therapy under microgravity conditions for the future studies.