Evaluation effect of silver nitrate on antioxidant activity and antioxidant compounds in calli frome leaves of lavandula angostifolia

Lavender with scientific name of Lavandula angustifolia belongs to Lamiaceae family ¡all parts of this plant are rich of secondary compounds with high antioxidant properties and can be a good source for providing antioxidants in tissue culture conditions.In this research with the aim of increasing plants secondry methabolites with high antioxidant properties ,silver nitrate as abiotic elicitor used in tissue culture of Lavandula angutifolia. Concentrations of 1, 2, 4 and 6 mg/l of silver nitrate as abiotic elicitor added to the one-month calli from leaf explant grown in MS medium with , Naphthaleneacetic acid(NAA)2mg/l, Benzyl amino purine (BAP) 4mg/l.After a month calli were harvested and the analyses were performed.The results showed that with increasing concentration of silver nitrate in the culture medium, growth of calli was increased and increasing the amount of silver nitrate concentration up to 4mg / l, increased Rosmarinic acid, flavonoids ,phenolic compounds and antioxidant activity .But in higher concentration of 4 mg / l the toxic effects of silver ions revealed and reduced the amount of Rosmarinic acid,phenolic compounds and antioxidant activity.