The Cytotoxic Effect of Chabahar Coast Marine Algae Gelidiella Acerosa Organic Extracts on Breast (MCF-7) and Colorectal (HT-29) Cancer Cell Lines

Marine algae are one of the natural resources which produce a wide range of new secondary metabolites with various biological activities such as antitumor and anticancer properties. The present study was conducted in order to evaluate the invitro cytotoxic effect of marine algae Gelidiella acerosa organic extracts against breast (MCF-7) and colorectal (HT-29) cancer cell lines.
In this laboratory study, cell lines of breast (MCF-7) and colorectal (HT-29) were cultured in RPMI medium with 10% fetal bovine serum (FBS). The cytotoxic effect of different concentrations (125, 250, 500 ,and 1000 μg/ml) of algae extracts cultured cells were evaluated by MTT (3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyltetrazolium bromide) and trypan blue assay. DNA fragmentation activity of the cells was evaluated by the 2% agarose gel after cell culturing by the different concentration of the algae extracts, trypsination ,and precipitation by RNAase. Data were analyzed using one-way ANOVA followed by Tukey post hoc test.
Survival of cancer cells in both methods was inversely correlated with the concentration and the percentage of survival decreased with increasing concentration. Concentration of 1000 µg/ml of methanol extract showed the greatest effect compared with the controls (p In this study, results showed seaweed extract can be the basis for further studies in order to recognize the chemical compounds of the extracts. Also, after invivo, preclinical, and clinical studies the extract can be used in food and pharmaceutical industries.