Quantitative Changes of Trigonelline Metabolite in Fenugreek (Trigonella foenum-gracum L.) Sprouts under Chitosan and Water Stress Induction

Application of bio-stimulants is one of solutions to quantitative and qualitative changes in metabolites of medicinal plants sprouts.
The aim of this study is to investigate the effect of chitosan bio-stimulation on trigonelline content of sprout under water stress.
This study was conducted in three separate factorial experiments (three times of 4, 8 and 12 days after planting) based on completely randomized design with 15 treatment in 3 replications. The treatments were chitosan in five levels (0, 0.05%, 0.1%, 0.2% and 0.4%) and water stress in 3 levels (control without stress treatment, -0.2 and 0.4 Mpa).
The result of variance analysis showed that chitosan, under stress and interaction effect of chitosan and water stress had significant effect on trigonelline content of 4, 8 and 12-day sprout. The trigonelline content decreased during the experiment in a way that the highest content of that was observed at the first days of seed germination.
By induction of water stress and application of chitosan with optimum amount (to 0.2%) decrease in trigonelline content attained to the lowest trend during the experiment.