Effects of 2-phenoxyethanol (2-PE) anesthesia on some haematological and biochemical indices of silver carp (Hypophthalmichthys molitrix)

In this study, the anesthetic effects of 2-phenoxyethanol (2-PE) on possible primary (cortisol level) and secondary (haematological indices and glucose level) stress responses were studied in silver carp (Hypophthalmichthys molitrix). Fish were first exposed to 0.1, 0.3, 0.5, 0.7 and 0.9 mL L-1 2-PE, and the time of induction (deep anesthesia) and recovery were measured. At concentrations of 0.5, 0.7 and 0.9 mL L-1 2-PE, all fish were anaesthetized within 3 min of exposure. For assessing possible stress effects caused by effective concentrations of 2-PE, the haematological indices, serum cortisol and glucose were determined in the deeply anaesthetized fish as stress indicators. 2-PE exposure resulted in a significant increase in red blood cell (RBC) amounts at 0.1 and 0.3 mL L-1. A parallel increase in hemoglobin and Haematocrite amounts at 0.1 concentrations (p