Effects of essential oils of Satureja bachtiarica and Nigella sativa on the efficacy of lactococcosis vaccine in rainbow trout (Oncorhynchus mykiss)

Lactococcosis has been defined as acute septicaemia, which causes economic losses in farmed fish, especially in rainbow trout. This study was done to evaluate the effects of the essential oils of Satureja bachtiarica and Nigella sativa on the efficacy of lactococcosis vaccine in rainbow trout. A total number of 270 fishes with a mean weight of 120 g were obtained; they were randomly divided into nine groups, each with three replicates, after two weeks of adaptation. The groups were: no injection group, vaccine only group, DMSO injection group, vaccine with 50, 100, and 200 micrograms Intraperitoneal injection (IP) injection. Two, four, and six weeks after vaccination, serological and haematological parameters were evaluated. In the sixth week, 1.7×107 cfu as LD50 96 hrs of Lactococcus garvieae were IP injected and the relative survival percentage was calculated. The results indicated that N. sativa essence is effective on the leukocyte population as the highest number of leukocytes were found in fish receiving high concentration of N. sativa. The relative survival rate of the studied fish decreased with decreasing concentrations of the N. sativa essential oil concentration, with a significant difference with control groups (p