Antibiotic resistance pattern and prevalence of tst gene in Staphylococcus aureus isolated from respiratory system infections in Isfahan

One of the most common infectious diseases in the world, is a respiratory infection that caused by attack of Staphylococcus aureus to the upper respiratory system. Due to the genomic diversity of species and toxins of Staphylococcus aureus strains and increasing antibiotic resistance of this bacterium, rapid detection of bacterium to reduce the transmission and spread of this pathogen in nosocomial infections is essential. The aim of this study was to survey the prevalence of tst gene and detection of antibiotic resistance pattern of Staphylococcus aureus isolates in respiratory system infection.
In this study 100 Staphylococcus aureus isolates from patients with respiratory infections were selected and the tst gene was proliferated with polymerase chain reaction and specific primers in this isolates were evaluated through gel electrophoresis. Study of the antibiotic resistance pattern of strains was done using the disk-diffusion method against 12 antibiotics, based on CLSI protocol.
In 14 of the 100 isolates studied, tst gene was identified. Most antibiotic resistance in this strains was oxacillin (100%) and the lowest antibiotic resistance was vancomycin (14.3%).
for producing different toxins in Staphylococcus aureus strains and the increasing antibiotic resistance of this bacterium, early diagnosis and appropriate treatment to prevent the progression of the disease caused by this bacterium is essential.