Evaluation of SLC30A8 Gene Polymorphism (rs13266634) Distribution in Patients with Diabetes Type 2 in West Mazandaran - Iran

Type 2 diabetes mellitus is a complex heterogeneous group of metabolic disorders characterized by increased levels of blood glucose due to impairment in insulin action and/or insulin secretion. The SLC30A8 gene is considered as one of the most important candidate genes of type 2 diabetes. The objective of this study was to evaluate the distributuion of this gene among our patients in Mazandarn, Iran.
Subjects and Mehtods: In this case-control study, 135 patients and 76 healthy subjects were recruited. Five ml of peripheral blood of diabetics and healthy subjects (controls) was collected in EDTA-containing tubes. Genomic DNA was extracted using salting-out method and genotype and allele frequencies of the rs13266634 polymorphism were investigated using PCR-RFLP method.
Genotype and allele frequencies of the rs13266634 polymorphism differed significantly between type 2 diabetic patients and non-diabetic subjects (P = 0.0003 and P = 0.0664 , respectively). The frequency of C allele was 89% in type 2 diabetes group and 67% in non-diabetic subjects, and this allele was significantly associated with type 2 diabetes risk (OR = 10.0, 95% CI 1.9124 – 591.3058 , 2.924 – 924.239).
Our results confirm the association between the rs13266634 polymorphism of the SLC30A8 gene and the increased risk of type 2 diabetes among the West Mazandaran population.