Preparation and Evaluation of Composite Nanofibers Containing Vancomycin

Electrospinning of chitosan/poly ethylene oxide (CS/PEO) and poly caprolactone (PCL)/ CS/ PEO/ solutions with the addition of vancomycin to create nanofibers with antibacterial properties were examined.
Polymeric nanofibers including CS/PEO and PCL /CS/PEO/ vancomycin were produced by electrospinning method. The morphology of electrospun nanofibers was studied with use of scanning electron microscopy (SEM). Moreover, the chemical structures of the nanofibers were evaluated by FT-IR. The drug release of nanofibers was also investigated by UV-Vis spectrophotometry. The antibacterial activity of scaffolds was tested by two type bacteria including Streptococcus pyogenes and Staphylococcus aureus.
The SEM images indicated that the addition of vancomycin as much as 0/8%wt brings about the best nanofiber. Also, the morphology of electrospun nanofiber is dependent on the viscosity of the solution and the ratio of PCL/CS /PEO/vancomycin. According to the results of vancomycin releasing from nanofibers, the best results were obtained in the presence of PCL/ CS/PEO/0/8%wt vancomycin nanofibers as healing sample.
The result of antibacterial activity of nanofiber and drug releasing showed this nanofiber can used as an antibacterial coating to prevent the spread of infections in the wound surface.