Compare Antioxidant and cytotoxic effects of aqueous and hidroalcoholic extracts of Ruta graveolens on DU145 cell line

The prostate cancer is considered as the second most common malignancy in men after skin cancer. The alternative methods and novel compounds with lower side effects can be very important and critical for the patients due to high costs and many complications of radiotherapy, chemotherapy and invasive tissue surgery. Tant and phenolic compounds. The Sodab plant, Ruta graveolens, as a member of herbal drugs has a long consumption history in traditional medicine of Iran and different nations besides its significant therapeutic effects. The Sodab rostate cancer cells. Furthermore, the antioxidant effect and the amount of phenolic compounds of Sodab extracts were evaluated.
The Sodab plant was prepared from the Agriculture and Natural Resources Research Center of Isfahan. The next step was preparation of the water, hydroalcoholic and methanol extracts of this plant. The DU-145 cell line cultivation conditions were RPMI-1640 culture medium which contains 10% bovine serum, incubation with 5% CO2 and different concentrations of aqueous and hydroalcoholic extracts during 24, 48 and 72 hours of incubation. The MTT method was used in order to estimate the cells viability percent in the presence and absence of the extracts and the optical absorption was read with wavelength 540 nm by the ELISA device.
The cytotoxic effect of Sodab aqueous and hydroalcoholic extracts was weak on DU-145 cell line while the antioxidant activity and the phenolic content of this plant were significant.