The Effects of Antinociceptive and Anti-inflammatory Spinacia olerace Ethanolic Extract in NMRI Mice

Spinach has various pharmacological effects such as anti-inflammatory, antioxidant, and anti-stress effects. We aimed to investigate pain killing effects of ethanol essence of spinach leaf in adult male laboratory mice by formalin test and hot plate. This study was done on 36 adult male small laboratory mice (NMRI) race with 25 to 30 gr of weight. The animals were randomly divided into 6 groups of 6 members including control groups (healthy), experimental groups (receiving formal saline) and also three groups receiving doses of 150, 300 and 600 mg/kg body weight ethanol essence, and standard groups (receiving morphine) with a dose of 10 mg/kg body weight. After the treatment, the animals were studied by formalin test and hot plate with regard to reaction to pain. In addition, anti-inflammatory activity was investigated by test in the animal. The data was then analyzed by one-way covariance analysis and then by Tukey test. The ethanol essence of Spinacia oleacea L leaf decreased pain at the chronic phase of formalin test and hot plate. In addition, ethanol essence of spinach leaf had significant anti-inflammatory activity in ear edema test. The ethanol essence in spinach leaf hasantinociceptive and anti-inflammatory activity in mice.