Evaluation of relationships of D, S and U genomes of Aegilops genus with A genome of Triticum using ISSR molecular marker

The objective of this study was to investigate the relationships between D, S, and U genomes in three diploid species of Aegilops genus with genome A in two diploid species of Triticum genus, using 15 ISSR primers. The ISSR primers were able to identify 105 locations, that the number of 6 locations were monomorphic and 99 locations were polymorph. The mean number of generated location was 7.00 and the average number of polymorphic location was 6.60. The primers of IS13, IS6 and UBC865 were introduced as superior primers for determining of genetic variation in the studying populations. There was a high genetic diversity based on the used marker among the species of two genera and the high contribution of variance to interspecies than intraspecific showed that the species were completely differentiating according to the studied marker. The results of similarity matrix, cluster analysis and principal coordinate analysis were showed that the two species were separated from together and in the species of Aegilops genus, Ae. umbellulata (U genome) had the most distance with other species, Ae. strangulate and Ae. tauschii (D genome) had the lowest distance and falling in one group with Ae. speltoides (S genome) in the next stage. Therefore, the D genome showed a lower distance with the S genome and the U genome had the greatest distance with two other genomes based on studied markers. The A genome (Triticum genome) had the most distance with three Aegilops genome. But the U genome among genomes belonging to the Aegilops genus had a lower distance than A genome.