Quantitative investigation of EGFR and FGFR4 expression level in lung cancer patient in compare with normal

Lung cancer is the most common cancer in the world and is the leading cause of death from cancer in people around the world. The cancer begins through activation of some oncogenes and inactivation of tumor suppressor genes. The aim of this study is to quantitative evaluation of EGFR and FGFR4 genes expression level in blood samples of lung cancer in compare with normal people to investigate the role of these two genes as biomarkers during lung cancer diagnosis and screening.
In this study, we investigate expression level of two main biomarkers (EGFR, FGFR4) among 50 lung cancer patients in compare with 50 control people. Total RNA from Blood samples were extracted and cDNA is synthesized. The specific primers for detection of markers are designed and expression level of BRIP1, PALB2 in presence of gene GAPDH by using Real Time PCR method was quantitatively studied.
FINDINGS: Assessment of quantitative comparison showed that the two genes (EGFR, FGFR4) expression level increased significantly in lung cancer patient in compare with control population. Results showed quantitative increase of FGFR4 and EGFR genes with 4/46 and 3.03 fold respectively for lung cancer in compare with normal samples.
CONSLUSION: Based on this study results we could predict the expression level of (EGFR, FGFR4) gens in suffered patients quantitatively which could use as biomarker indicator during screening of lung cancer samples. So could be used in screening of population for early detection to inhibit cancer.