Cytotoxic evaluations of metanolic extract fractions from Haplophyllum tuberculatum against RAJI and A549 cancerous cell lines

Haplophyllum tuberculatum (Forssk.) A. Juss., is a perennial plant from Rutaceae which is a valuable folk medicinal plant. The leaves, stem and root samples of shadow dried plants gathered from Hormozgan province-IRAN, were extracted with methanol and total phenolics, Flavonoids and alkaloids contents were determined by spectrophotometer. Then the methanolic extract was fractionated by Di-ethyl-ether, Chloroform, Butanol, and Water respectively. The cytotoxic effects of fractionated extracts were evaluated against RAJI and A549 cancerous cell lines and intact blood lymphocyte cells by MTT assay. Results showed that the most phenolics were accumulated in stems and the most alkaloids and flavonoids contents were observed in the plant leaves. Di-ethyl-ether extract from all three plant organs had moderate cytotoxic effect (IC50