Effect of Silver Nanoparticles Produced by Paenibacillus on Rat Cutaneous Wound Healing

Silver nanoparticles have different biological effects and are used in treatment of burn infections, open and choronic wounds. In this study, the bacterial strains of Paenibacillus genus were isolated from soil samples in Damghan, Iran and identified using molecular method. After preparing the condition for silver nanoparticles production, the biological production was proved through spectrophotometry, transmission electron microscopy (TEM), and X- ray diffraction analysis (XRD). Then, wound induction was done on rats’ skin and the healing effect of nanoparticles was investigated in three groups of rats (treatment, control and phenytoin, n=8 per group). At days 3, 7, 14, and 21 after treatments, the wound area was measured in all groups. Afterwards, wound sampling was carried out for histological evaluations. Silver nanoparticles were produced in spherical shapes (40nm) by Paenibacillus genus. The rate of wound healing was faster in treatment group (the coverage texture was completely formed on day 14) compared to that in other groups. The silver nanoparticles produced had healing property effects which could accelerate wound healing.