Effect of Resveratrol Supplementation and Aerobic Exercise on the Protein SIRT1 and PGC-1α Skeletal Muscle and Subcutaneous Fat Tissue's UCP-1 Levels Wistar Male Rats

The purpose of this study was to determine the effects of resveratrol supplementation and Aerobic training on protein levels of SIRT-1, PGC-1α and UCP-1 soleus muscle and Ingoinal abdominal subcutaneous adipose tissue in adult male Wistar rats. In this study, 32 male rat (w=260흈, 8 weeks) divided into 4(n=8) groups: control(C), aerobic training (T), complement-training (T-R) and supplements(S) randomly. Training groups exercised for 12 weeks (5 sessions a week, each session 45 minutes on a treadmill. ELISA (sandwich double) was used to measure tissue protein UCP-1, SIRT1, PGC-1α. ANOVA test used for data analysis and statistical significance (P≤0.05) were considered. Increased SIRT1 and PGC-1α protein changes in T-R and S groups in muscle tissue and subcutaneous white adipose tissue, respectively (P≤0.001) and (P≤0.05). Showed that the protein UCP-1 is also a significant increase in subcutaneous white adipose tissue after workouts (P≤0.001). According to the results probably resveratrol supplementation with aerobic physical activity a stronger impact on increasing the SIRT-1, PGC-1α soleus muscle and inguinal adipose tissue and UCP-1 inguinal adipose tissue to use separately or supplement physical activity resveratrol and white fat and is likely to change subcutaneous white adipose tissue to beige or intermediate phenotype.