The Pattern of Expression of Tumor Necrosis Factor-? Induced by Hydro-Alcoholic Extract of Zingiber officinale

Inroduction &

Diabetes is known as an inflammatory disease associated with metabolic disturbances. Tumor necrosis factor- Alpha is one of the main inflammatory cytokines and plays a central role in host defense, inflammation and immune function. The use of various medicinal herbs, including ginger, has been used to reduce the complications of inflammation from the past. In this study, the effects of various concentrations of ginger hydro-alcoholic extract on TNF-α gene expression in bone marrow derived mesenchymal stem cells were investigated.
Material and

Mesenchymal stem cells were studied in control group and six groups of treatment with ginger hydro-alcoholic extract at concentrations of 100 and 50 μg / ml at 2, 16 and 24 hours. mRNA of cells were extracted and after cDNA synthesis, TNF-α gene expression was investigated using q Real Time PCR. Using the expression level, the results were evaluated by t-test.

Samples treated with ginger extract at a concentration of 100 and 50 μg / ml for 24 and 16 hours showed a significant decrease for TNF-α gene (p

According to the results, this decrease in the expression of TNF-α indicates the long-term effect of this extract compared to its short-term effect of 2 hour. Therefore, the effect of ginger on the expression of TNF-α inflammatory gene is dependent on treatment concentration and time.