Molecular identification and evaluation toxin production of Clostridium perfringens type D strains isolated cases of the enterotoxaemia in the Razi Institute

Clostridium perfringens is cause of enterotoxaemia disease in sheep. The purpose of this research was to select the best strain Clostridium perfringens type D isolated from Iran and their comparison with vaccinal strain by MLD and potency tests. In this research 15 strains Clostridium perfringens type D were studied. At first, microbiological and biochemical tests were done then specimens were confirmed by PCR test. Finally, the best strain was selected by MLD test and the enterotoxaemia vaccine was prepared by the selected and the vaccine strain separately. The vaccines were kept for 45 days at refrigerator then potency test was done according to standard protocols. The results showed that the conditions for epsilon toxin production by isolated strain and the vaccine strain was nearly similar.