The Effects of High Intensity Interval Training Preconditioning on Metabolites of NO (NO2- ,NO3-) and Infarction Size of Myocardium after Ischemia/Reperfusion Injury in Healthy Male Rat

The purpose of this study was the effects of exercise preconditioning with high intensity interval training (HIIT) on metabolites of NO (NO2- , NO3-), and infarction size of myocardium after ischemia/reperfusion injury in healthy male rat. For this reason, 44 wistar rat were randomly divided into 4 group HIIT (n=8), Group HIIT + protocol IR (n=14), control group (n=8) and control + protocol IR (n=14). Each session of HIIT was 1 hour of exercise in the 3-stage, 6-minute running of 50 to 60% VO2max warm up, 7 Period 7 minute running with a including of 5 to 20 ° slop (4 min with an intensity of 80 to 120, 3 minutes of 50 - 60% VO2max) and 5-minute running of 50 to 60% VO2max cool-down. Control group did not participate in any exercise program. NO and its metabolites were measured by using Griess reaction. Results showed there was a significant difference on nitrite (0.855 mol per liter, equivalent to 34.79%), nitrate (6.202 mol per liter, equivalent to 149.48%) and NOX (6.6 micromoles per liter, equivalent to 98.11%) levels in HIIT group than control group. That, the results showed, AAR was significantly more (9.06%, p=0.010) and INF was significantly lesser (23.2%, p <0.001) in exercise training group in comparison with control. In generally, it seems that increase in No- NO3-, NO2- axis is one of mechanisms that HIIT exercise program can protect heart from Ischemia/Reperfusion injury and decreased myocardial infarction.