Evaluation of antimicrobial properties of some in digenous lactobacillus plantarum strains isolated from goat,s milk

In this study the antimicrobial potential of Lactobacillus plantarum strains isolated from goat,s milk was evaluated. Sixty seven goat,s milk samples were collected from Tehran and Karaj and screened for the presence of lactic acid bacteria (LAB). Among the isolated LAB, 18 strains were identified as Lactobacillus plantarum by their carbohydrate fermentation pattern and 16S rRNA sequencing. Antimicrobial activity of the isolatedLactobacillus plantarum strains against a number of pathogens including Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhi, Listeria monocytogenes and Escherichia coli was evaluated by agar spot and well diffusion method. Results indicated 10 of theLactobasillusplantarum strains to possess inhibitory activity towards the tested pathogens, while 6 of these strains were shown to have wide spectrum of inhibitory activity and were able to inhibit the growth of the entire tested gram positive and gram negative pathogens. The antibacterial activity demonstrated by these 6 strains was further analyzed by subjecting their supernatant fluid to various physico-chemical treatments. The supernatant fluid of these strains retained their antagonistic action even after neutralization, while only 2 strains (LP4 and LP14) retained their antimicrobial activity after treatment with 1 mg/ml catalase. The supernatant fluids of the respected isolates LP4 was resistant to enzyme Lysozyme and lipase, while proteolytic enzymes like pepsin, trypsin and proteinase K completely destroyed its antibacterial potential. The isolates were resistant to their own antagonistic action. The bacteriocin like substance produced by LP4 was pH and heat resistant as it resisted wide pH range of 3-8 and autoclave temperatures of 121ºC for 15 min. This substance after 4 weeks storage at -20 0C still retained its antimicrobial activity and after treating by concentration of 7 percent NaCl, it was still active.