Assessment the Wound Healing Efficiency of the Microbial Produced Alginate and the Extract of Persian Mannaplant in the Rat Wounds: the Complex of the Modern and Traditional Dressings

Achievement the new biocompatible wound dressing is one of the attractive areas of research. The present study attempts to examine the healing effects of the alginate - Persian mannaplant extract in the induced rat wounds. After culturing and detecting of the Pseudomonas aeroginosa strains by phenotyping and genotyping methods, the produced alginate was extracted and used for cell cytotoxicity assessment by MTT assay. 1.5×1.5 cm wounds were made on the tested rat skins. The animals were divided in 4 groups (n= 8). Three groups were equally treated for 21 days with nontoxic doses of alginate hydrogel, herb extract, and alginate hydrogel- herb extract, respectively. The forth group remained as the negative control. In different days after treatments 2 rats from each group were selected and the wound areas and the effects of each material were analyzed. Alginate was extracted from P. aeruginosa strain K1. Results from the macroscopic examination showed that the wound contraction percentage in alginate hydrogel and alginate hydrogel- Persian mannaplant groups had significance difference with the rest other groups
(P value < 0.05). Microscopic examination showed that the best group was the one which was treated by alginate hydrogel- Persian mannaplant complex. Although both materials had a good ability to heal the wounds but microscopic examinations showed that the alginate hydrogel- Persian mannaplant complex had better activity in the wound site.