Effects of triploidization by heat shock treatment on growth performance and amino acids profiles in rainbow trout (Oncorhynchus mykiss)

The induction of triploidy is an effective strategy for the production of sterile fish for aquaculture and has usually been reported to be accompanied by modifications in physiological characteristics. The aim of this study was to assess the effects of triploidy induction on growth performance and amino acid profiles in rainbow trout. Eight female (total weight 1600 ± 246 g) and six male (total weight 1393 ± 186 g) four-year-old healthy rainbow trout were used in this study. Triploidy was inducted through the application of heat shock of 28 ºC for 10 min to eggs 10 min post fertilization in an aquarium equipped with a heater. During the 38 days of rearing period, the fish were fed a rainbow trout commercial diet (BioMar, France) 12 times a day at the rate of 7% of their body weight. Fish ploidy level was determined by erythrocyte size measurement. Based on red blood cell analysis, the overall triploidization success level was 87.1%. Growth performance was significantly higher in diploids as compared to triploids (p<0.05). The levels of non essential amino acids increased and the levels of essential amino acids decreased as an effect of triploidy induction. Triploidy induction in rainbow trout affects growth performance and amino acid profiles in rainbow trout.