Establishment and characterizations of a new cell line from larval hemocytes of rose sawfly Arge ochropus (Hymenoptera; Argidae)

We established and characterized a hymenopteran cell line from Arge ochropus for the first time. The established cell line was named AOU-G, standing for Arge ochropus University of Guilan. This cell line was derived from larval hemocytes of the rose sawfly whose characteristics and susceptibility to 20-hydroxyecdysone have been verified. We also noticed four types of hemocytes such as prohemocytes, plasmatocytes, granulocytes and oenocytes. The population doubling time of 20th passage of this new cell line in EX-cell or Grace medium at 28˚C was 3 and 5 days, respectively. Biochemical studies in rose sawfly hemocytes showed considerable changes in some component: protein, glucose, cholesterol and urea. Except urea, all components were significantly reduced. We suggest that the AOU-G cell line as a productive new cell line that could be very useful in studies concerning bio- insecticides, immunology, endocrinology and toxicology.