Cellular toxicity, hemolytic and anticoagulant effects of Siphonaria carbo limpet extracts

The present study was conducted to study cytotoxicity of extractions from Siphonaria carbo limpet. Brine shrimp lethality assay, hemolytic effects on erythrocytes and coagulation tests was performed using aqueous and ethanolic extracts. Sampling was performed from Bushehr coastal rocks. Extraction was carried out using wet and dry tissue. Results of toxicity assay on Artemia nauplii showed lethality effects of extracts, except for dried aqueous extract. The lethality of extracts was in correlation to concentration. Wet aqueous extract showed the highest toxicity on tested cells (LC50= 5.98 mg/ml) in contrast to other extracts. The results of hemolysis test revealed no significant hemolytic activity of extracts. The results of anticoagulant assays did not confirm any anticoagulant effects in any of extracts. In conclusion, in spite of the negative results of hemolytic and anticoagulant effects, the cytotoxicity of the extracts might be utilized for further anticancer and antiparasitic screening.