Prevalence of human Cryptosporidiosis by Polymerase Chain Reaction method in Varamin city in 2011 -2012

Cryptosporidiosis is one of the more current cause of diarreheal disease in humans and many mammals which intestinal epithelial cells infect with protozoal agents cryptosporidium. This study was done to evaluate epidemiological prevalence of the human cryptosporidiosis in Varamin. Study was done as cross- sectional in 2011 -2012 including 930 individuals with diarrhea (530 males ,400 females ) ranged between 1 to 60 years (27±2 mean age) admitted to Mofateh hospital. Stool samples were collected and stored at 4°C in potassium dichromate until processing. Cryptosporidium oocysts were detected and identified by optical microscope using concentrated formalin detergent and modified Ziehl-Neelsen staining methods. DNA extraction was done and samples were confirmed by specific primers and PCR method. The infection rate in age group1-10 years was 12 cases (5.17 % ), 7 cases (3.74 % ) in 11-20 years ,8 cases (4.14 % ) in 21-30 years ,4 cases (3.41 %) in 31-40 years ,3 cases (2.43 %) in 41-50 years and 1 case (1.28 %) in 51-60 years which significant correlation was seen between cryptosporidial infection and age (P < 0.05 ). DNA band was 555 bp. Direct or indirect relationship between livestock and human could play an important role in crytosporidiosis transmission, so hygienic principles are necessary.