The Study of Biofilm and slime formation coded by icaA and icaD genes in Methicillin resistant Staphylococcus aureus containing mecA gene in Shahrud County (1396-1397)

Currently, scientists hold the conviction that attachment and biofilm formation are theinitial factors in bacterial pathogenesis. The present study aimed to investigate the coexistence oficaA and icaD genes involved in the slime and biofilm production of methicillin-resistantStaphylococcus aureus containing mecA gene and their relations to its pathogenesis. In this descriptive cross-sectional study, 100 wound samples werecollected from one of Shahruod hospiltals in 2017. The isolation and purification of Staphylococcusaureus was performed. It should be noted that resistance to methicillin was investigated by KirbyBauer test. The study of slime production and biofilm formation were carried out using Congo redagar and 96 microtiter plate-based methods, respectively. The examination of the relationshipbetween mecA, icaA, and icaD genes was performed by Multiplex polymerase chain reaction. In this study, 65 methicillin-resistant Staphylococcus aureus samples were isolated of which67% and 66.6% were able to form strong slimes with black colonies and strong biofilms,respectively. The coexistence of mecA, icaA, and icaD in 89.23 of the samples was observed. According to the results of the present study, there was a relation between pathogenesisand antibiotic resistance in methicillin-resistant Staphylococcus aureus, which should be consideredfor the treatment of the infection caused by this bacterium