Investigating the antibacterial effect of wound healing with Henna extract:

Wound environment is susceptible to growth of microorganisms and infection. To prevent the infection and accelerate the process of wound healing, antibacterials are sometimes required. The purpose of this study is to develop a bacterial resistant wound dressing based on Henna , a natural bioactive agent. After separating impurity of Henna leaves, they were milled and dissolved in ethanol. In the next step, cotton or polyester fabrics were impregnated with filtered ethanol extract of Henna leaves using exhaustion method. The antimicrobial properties of cotton and polyester fabrics were quantitatively measured by the standard penetration test (AATCC 100-190) against Staphylococcus aureus as gram-positive and E. Coli as gram-negative bacteria. The findings show that the cotton fabric impregnated with Henna extract has an acceptable antibacterial properties against gram-positive bacteria with poor antibacterial effect against gram-negative bacteria. Moreover, the polyester fabric treated with Henna extract did not show any antibacterial properties against both gram negative and gram-positive bacteria. This was attributed to the high temperature which was used for treatment of polyester fabrics with Henna extract.