The Effect of Hydroalcoholic Extract of Cinnamomum Zeylanicum on Changes of Ovarian Tissue and Hormone in the Cyclophosemide Treated Rats

  The use of cyclophosphamide in the treatment of some diseases with its adverse side effects is imperative. Considering the well-known value of cinnamon extract, the present study aimed to evaluate its protective effect against the effects of cyclophosphamide on ovarian function in mice forty-nine adult rats were arranged in seven groups. No treatment control group, sham group was injected drug’s solvent, and treatment groups 1, 2, 3 were injected 5mg/kg/b.wt Cyclophosphamide daily. These groups were gavaged )0.5, 1, 2(mg/kg/b.wt Cinnamon extract daily. Group 4 was injected 5 mg/kg/b.wt Cyclophosphamide, and Group 5 was gavaged 2 mg/kg/b.wt Cinnamon extract. After 21 days, all rats were unconscious and blood was taken from their hearts. Immediately, the left ovary of them was removed and after fixation in 10% formalin solution and tissue processing, the sections with 5 micron thickness were prepared and studied. By centrifuging the blood, serum was isolated and the concentration of FSH and LH, estrogen and progesterone hormones was measured by ELISA method. The obtained data were analyzed with SPSS (18( and ANOVA, and they were compared with Duncan’s test. The study of prepared photomicrographs of ovarian tissue indicated that the mean of follicle types and corpus luteum presented a significant decline in the treatment of Cyclophosphamide and Cinnamon extract especially in maximum dose compared to the control group and increase compared to the treatment Cyclophosphamide (P<0.05). Compared to cyclophosphamide treatment, there was a significant increase in the level of P <0.05. Extract of cinnamon extract increased significantly compared to other groups. Significant increase in atherosclerosis was observed in cyclophosphamide treatment and its reduction in other treatments. Significant decrease in FSH and LH, estrogen and progesterone in cyclophosphamide treatment and their increase in other treatments were observed.

According to these results, the cinnamon extract with its antioxidant properties had a dose- dependent protective effect on the pituitary-gond axis, and by increasing the number of follicles and the level of ovarian hormones, it reduced the adverse effects of cyclophosphamide in the ovary. Therefore, further research is recommended on generalizing this result to humans.