Isolation and identification of Vibrio bacteria in the black- lip pearl oyster and their sensitivity and resistance to antibiotics in the West Persian Gulf

The black-lip pearl oyster, Pinctada margaritifera, is one of the important species of Mollusca which has been cultured to produce pearl. Mortality occurs in all stage of black-lip pearl rearing, but few studies have been conducted on this issue. The aim of this study is to investigate the pathogens of black-lip pearl oyster rearing site on Lengeh Port and also determine bacterial resistance and antibiotic sensitivity of the identified bacteria. Fresh samples were used for bacterial analysis. The bacterial analysis was done according to biochemical methods and bacterial Gram staining and using TSA and TCBS culture medium. Bacterial isolates were studied using Mueller Hinton Agar medium for the investigation of the resistance and antibiotic sensitivity of bacteria. Four different species of the genus Vibrio bacteria were found that Vibrio harveyi, Vibrio alginolyticus, Vibrio splendidus, Vibrio anguillarum were in the gills of black-lip oyster. Bacteria were resistant to ampicillin, erythromycin, streptomycin and oxacillin, sensitive to novobiocin, and intermediate to oxytetracycline.