Evaluation of the Ability of Retrotransposon Markers to Study the Genetic Diversity in some Populations of Radish (Raphanus Sativus)

Radish belonging to the Brassicaceae family, contains some componds such as cineol, malvin Chloride, Sapogenin, Sulforaphane and Raphanin and has many therapeutic properties. In this study, IRAP retrotransposon marker was used for evaluation of genetic diversity in seven commercial and native populations of Radish herb. The least number of bands (1) were produced by LTR2 & LTR15 (primer combinations) and the highest number, (16) were produced by LTR23 primer. The mean number of bands in all sites was 6. 39. the most diversity index (0.41) was found for Sukkula primer and the least diversity index (0.18) was related to LTR2-LTR15 primer and also the mean diversity index was 0.28. The highest marker index (4.1) was related to the LTR3 primer and the least marker index (0.18) was related to the LTR15 and LTR2 primer combination. The most number of effective bands, the Shanoon diversity index and the Nei diversity index were 1.82, 0.64 and 0.45, respectively and all belonged to Sukkula primer. The least number of effective bands, the Shanoon diversity index and the Nei diversity index were 1.18, 0.27 and 0.15, respectively belonged to the rtdk primer. Among radish populations, the most percentage of polymorphic location, effective band, the Shanoon diversity index and the Nei diversity index were 46.85, 1.305, 0.17 and 0.26, respectively. Molecular variance analysis among and between populations showed that 59% of the total genetic diversity was among populations. The results of this study showed that the population of Profit could be used as a parent or maternal parent or gene selection provider in Iran's radish breeding, since it showed the most polymorphism, the Shanoon diversity index and the Nei diversity index and number of effective bands.