Studying of different salinity concentrations on the growth microalgae "chlorella sp" from rice fields Golestan Province

Salinity is one of the most important factors affecting growth, photosynthesis and valuable metabolites regarding large scale cultivations. The aim of this research was studying the effect of salinity on growth and pigment composition of Chlorella sp. Collected from paddy-fields of the Golestan Province for evaluating of the potentiality for large scale cultivation. Purification was done using N8 culture media at 2 uE.m-2.S-1 irradiance, 280C and continuous illumination at pH 7.2. Salinity treatments were Sodium Chloride at 0, 0.25.0.5 and 1% ( 17 to 170 mM) concentration. Survival and growth were assayed by turbidity, pigment composition as in vivo and in vitro were measured after normalization at the dry weight unit. Results showed that the sharp increase in growth rate viewed at 5th day after inoculation at both control and treatments. Survivality would be keep at this salinity range. The optimum of growth and the highest degree of special growth rate were seen in 0.5% salinity. Difference between the growth at 0.5 and 1 % salinity were significant. The ability of carotenoide production at the 9th day after inoculation were significantly more than the other treatments and insignificant for chlorophyll production. The results show that Chlorella sp., have acclimatized potentiality against this degree of tension and regarding that would be considered as a suitable candidates for future bio fertilizer and soil conditioner inoculation in Golestan province.