Study of some environmental factors on seed germination of Lepyrodiclis holosteoides

Lepyrodiclis holosteoides is a weed from Caryophyllaceae family that has infested some of wheat and canola fields. There is not suffiecient information about the effect of environmental factors on seed germination of this weed. Therefore, the effect of some factors on seed germination of L. holosteoides was studied in this research. The effect of temperature (18/10, 25/20, 30/21, and 30/25◦C), scarification with concentrated sulphuric acid (1, 3, 6, 9, 12, 15 min), gibberelin (0, 10, and 20 ppm), salt stress with NaCl (0, 50, 100, 150, 200, 250, 300, and 350 mM), and drought stress with PEG (0. 15.6, 100, 151.4, 190.5, 223.6, 279.3, and 326.6 gr/100 ml water) was investigated on L. holosteoides seed germination. In greenhouse, effect of planting depth (1, 3, 6, 9, 12, and 15 cm) was studied on seedling emergence percentage. The results showed that optimal temperature of seed germination was 25/20◦C. The maximum (80%) and minimum (44%) seed germination was due to 1 and 15 min scarification with sulphuric acid, respectively. Gibberelin did not affect seed germination. Lepyrodiclis holosteoides germinated only to response to 50 and 100 mM NaCl, and PEG 15.6, 100 gr/100 ml water. The best planting depth was 1 cm. This study concludes that L. holosteoides distribution is more limited to moderate climate and it is not a problematic weed in warm regions.