Production of conjugated linoleic acid nano lipid carrier (NLC) for enrichment of low-fat pasteurized milk by it.

The use of nano-carrier for hydrophobic nutraceutical compounds such as essential fatty acids have multiple benefits such as controlled release in foods and in the body in a certain time and place. In this research production and evaluate the optimal oral nano lipid carrier containing CLA and adding it to low-fat pasteurized milk to make a product enriched with functional bioactive compounds had been discussed. The hot-homogenization technique was applied to produce NLC dispersions. NLCs prepared at 6% (w/v) poloxamer 407 concentration and at 10:1 ratio of cacao butter (solid lipid) to CLA (liquid oil) showed low particle size and narrow particle size distribution (81 nm and span = 0.83, respectively).The results of various testes such as particle size, stability over a period of storage time (60 days) (93 nm), turbidity and zeta potential were showed good physicochemical properties of nano lipid carrier. TBA test also showed CLA nano lipid carrier had lower percent of malon aldehyde and so higher stability against oxidation during 60 days storage. (p<0.05). Assessment of remaining CLA in pasteurized low fat milk fortified with CLA loaded nan lipid carrier during 14 days sampling showed that use of nano lipid carrier protected CLA during heat treatment (3.9% of fatty acids in milk) and we can use this system for protect CLA against oxidation, heating and other treatments to use this system for enrichment low fat dairy products.