Effect of lead and putresine interactions on cress (Lipidium sativum) seedling physiological and biochemical factors

In the present study, the effects of lead and putrescine on Lepidium sativum plant were examined. A factorial experiment was conducted in a completely randomized design with four replications. Lead nitrate at concentrations of 0, 125, 250 and 1000 micro molar and putresine at 0 and 1 mM was used. Lead and putresine increased plant fresh weights compared to lead treatment of same concentration and at a concentration of 500 micromolar fresh weight was10 gr. There was no significant difference between dry weights of treated plants and control. Lead treatment increased polyphenol oxidase (0/8 micromoles per minute per gram fresh weight in 1000 micromolar) and catalase (0/053 micromoles per minute per gram fresh weight in 1000 micromolar) activities. The use of putrescine and lead increased the polyphenol oxidase enzyme activity (0/99 micromoles per minute per gram fresh weight in 125 micromolar) compared to lead treatment of same concentration. The combined use of putresine and lead reduced the amount of proline except at 250 micromolar of lead compared to plants treated in the same concentration of lead. Treatments of lead at concentrations of 125, 250 and 500 micromolar and putrescine, were reduced the percent of inhibition compared to the treatment of lead in the same concentration. Inhibitory percentage was increased on 1000 micromolar of lead. The total phenols were not significant difference under the lead and putrescine treatments. In Lepidium sativum under lead stress activated polyphenol oxidase, Proline increasese. Useing putrescine has controlled production of proline by reduction the stress effects