The Effect of Ethanol Extract of Rhizome of ( Alpinia officinarum )With 17 -Methyl Testosterone on Sperm Quality Parameters in Adult Male( Pocillia latipinna )

In this study comparison between effect of 17 -methyl testosterone and galangal extract on male Pocillia latipinna reproductive system and adrenal hormone(cortisol ) were investigated . For this purpose after dechlorinated the water of aquariums , 100 mature fishes with mean weight 3-4 g were released in aquariums . Adaptation of fishes for 48 hours were done . Then physicochemical factors such as water temperature , pH , water hardness and (DO) were determined . The experiments were performed in 10 groups (2 control and 8 treated groups ) , each group consisted of 10 pieces of fishes that were male and mature (were injected 0.02 ml every time. Main fish treatments recevied 4 doses of galangal extract With 10 , 20 , 30 and 50 mg/ kg and 17 -methyl testosterone with 1,5,10,20 mg/kg . Injection were intra muscular under dorsal fin of fishes , Injections were done in every other day for 20 days . Then fish speciments were Killed . Their testes were removed and fixed in 10% formalin . Biometry of length and weight of fishes were measured . Then testes were removed and histological structures of testes were compared with
control treatments. Results showed the gonadosomatic index was increased in 30 and 50 .