Mechanism of estradiol effects on adenocarcinoma colorectal (HT29) cell death and metastasis

Studies have shown that sex steroids affect on digestive system cancers. The aim of this study was to investigate the effects of cytotoxic dose of estradiol on BAX, Bcl2 and CD82/KAI1 genes expression in adenocarcinoma colorectal cells (HT29).

In this laboratory-experimental study, HT29 cells were divided into the control group and a group exposed to cytotoxic dose of estradiol (0.1 mg/mlfor HT29). Real-time PCR was used to evaluate BAX, Bcl2 and CD82/KAI1 genes expression levels. The data were statistically analyzed between groups using ANOVA.

In HT29 cells exposed to cytotoxic dose of estradiol, BCL2 and BAX genes expression levels significantly increased and decreased, respectively (p<0.001). CD82/KAI1 gene expression level significantly increased (p<0.01).

The estradiol cytotxic mechanism of action in adenocarcinoma colorectal cells is BAX- independent cell death. The cytotoxic dose of estradiol also enhances metastasis inhibition in adenocarcinoma colorectal cells.