Antidiabtic and Hepatoprotective ‎Effects of Hydroalcoholic Extract of ‎Trifolium pratense in Mice

Recent reports have shown that some Plant Isoflavones have beneficial effects on diabetes and liver health in human and laboratory animals. The aim of this study was to administrate of hydroalcoholic extract of Trifolium pretense (red clover) as a plant rich in Isoflavones compounds and its effects on serum levels of glucose and liver enzymes (AST, ALT and ALP) in mice. In this experimental study, 42 male mice were divided into 6 groups of 7, including healthy control, diabetic without treatment (positive control), diabetic treated with glibenclamide groups, and three diabetic groups treated respectively with 250, 500 and 750 mg/kg of hydroalcoholic extract of red clover by gavage for 15 days. Diabetes was induced by intraperitoneal injection of streptozotocin, 60 mg/kg. On the last day, serum glucose and Liver Enzymes (ALT, ALP and AST) levels were measured. The data were analyzed by SPSS statistical software and OneWay ANOVA test. The significant was shown with (P<0.05).Doses of 500 mg/kg and 750 mg/kg of extract were significantly (P<0.001) decreased serum glucose compared with positive control group.  Assessment of liver enzymes shown that AST level in 750mg/kg group (P<0.001), ALT level in 500 mg/kg (P<0.01) and 750 mg/kg (P<0.001) groups, and ALP level in 250 mg/kg (P<0.05), 500 mg/kg (P<0.001) and 750 mg/kg (P<0.001) groups significantly decreased compared with positive control group. It seems that the red clover has anti-diabetic and hepatoprotectiveeffects.