Investigation of the presence of G354A (Cys87Tyr) mutation in osteoprotegerin gene in women with osteoporosis in Chaharmahal and Bakhtiari province

Osteoprotegerin (OPG) is a competitive inhibitor of the differentiation,activity of osteoclasts,which inhibits thefinal stages of osteoclast formation,induces its apoptosis. In addition,OPG is considered as one of the most important candidate genesin the pathogenesis of bone diseases such as osteoporosis,idiopathic hyperphosphatasia. The G354A (Cys87Tyr) mutation in the OPGgene leads to idiopathic hyperphosphatasia. This mutation is probably related to osteoporosis. The purpose of this study was to investigatethe presence of G354A (Cys87Tyr) in women with osteoporosis in Chaharmahal,Bakhtiari province.

In this descriptive-analytical study,the bone mineral density (BMD) of the femoral neck,lumbar spine of women referring toShahrekord bone densitometry centers was measured by the X-ray absorptiometry technique in 2013-2014. Based on T-scores,people withosteoporosis were identified,70 patients were enrolled in the study after receiving their consent. Finally,DNA was extracted from bloodsamples,amplified by polymerase chain reaction (PCR) technique,and sequenced by DNA sequencing method.

After DNA extraction from the blood,the quality,quantity were determined by gel electrophoresis,spectrophotometry,respectively. Then,the gene was amplified by the PCR method,the product was detected by gel electrophoresis,followed by sequencingthe samples to investigate the presence of the mutation. Eventually,genotypes associated with Cys87Tyr mutation were not observed inthe studied population.

In the present study,the G354A (Cys87Tyr) mutation associated with idiopathic hyperphosphatasia was not found in womenwith osteoporosis