Iridoid and Furanolabdane -Type Diterpene Glycosides from Rhizomes of Eremostachys azerbaijanica Rech. f.
The rhizomes of Eremostachys azerbaijanica Rech. f.,as an indigenous plant in East Azerbaijan province of Iran, were studied for isolation and identification of possible phytoconstituents.
The air- dried and ground rhizomes were extracted with n-hexane, dichloromethane (DCM) and methanol (MeOH) using a Soxhlet apparatus. The 10%, 20% and 80% MeOH in water C18 cartridge solid phase extraction products )Sep-Pak fractions( of the MeOH extract were subjected to preparative reversed- phase high performance liquid chromatography (RP-HPLC) and the isolated pure compounds were identified by one- dimensional nuclear magnetic resonance (1D-NMR) spectroscopic technique.
The spectroscopic data of the compounds were compared with the respective published data and the obtained results showed the presence of four pure components, 6 - Hydroxy loganin (1), Shanzhiside methyl ester (2), Eremostachiin (β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl ester of phlomisoic acid) (3) and Phlomisoside II (4), with iridoid and furano labdane- type diterpene glycoside structures.
The occurrence of these types of chemical structures might be a confirmation to close relation and similar pharmacological and biological activities between Eremostachys and Phlomis genera.
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