Application of Dermal Fibroblast Cells in Cell Therapy of Wound

Cell therapy, with autologous dermal fibroblasts, shows remarkable potential and is currently achieved by different methods, such as spraying, grafting, and injecting to treat various types of ulcers, wrinkles, acne scars, and surgical wounds.

To prepare fibroblasts for clinical use, some critical points must be taken into consideration: isolation and culture of these cells to produce a homogeneous population of non-contaminated cells, a method for rapid growth, and development of a proportional number of fibroblast cells with proper strength and viability. In addition, an appropriate site for a biopsy should be selected based on indications of treatment and maintenance.

Ultimately, the clinical use of fibroblasts in wound healing obviates the need for ready to use reservoir of these cells. In this regard, dermal allografts can be used in both fresh and frozen forms. The fresh type is more desirable due to its high chance of survival. Developing the appropriate preservation methods for allogeneic fibroblasts is the emerging research field of interest. Furthermore, applying biomaterials will increase the efficacy of fibroblasts in wound healing.